OBTAINING SPECIFIC COMPONENTS FOR THE MANUFACTURE OF ERYTHROCYTE DIAGNOSTICUM FOR CLOSTRIDIAL INFECTIONS IN ANIMALS
УДК: 619:636.2:579:851.13:636.22 DOI: 10.31588/2413_4201_1883_2_255_302
Keywords:
clostridiosis, diagnosticum, IHA, specific antigen and serum КлостридиозыAbstract
Clostridiosis of farm animals is widespread in large livestock complexes of the Russian Federation. The disease causes significant economic damage to enterprises due to high mortality of sick animals, costs of treatment and preventive measures and reduction of animal productivity.
Among clostridiosis, the most common in practice is anaerobic enterotoxemia of young animals, resulting from rapid multiplication in the intestine of anaerobic bacteria Cl. perfringens serotypes A, B, C, D, E, F and absorption of the resulting toxins. As a result, animals develop catarrhal-hemorrhagic enterocolitis, intoxication, damage to the nervous system, often leading to death.
Currently, the diagnosis of clostridiosis of farm animals is carried out on the basis of the results of clinical, epizootological, pathoanatomical and laboratory studies. An enzyme-linked immunosorbent assay test system for serological diagnosis of clostridiosis has been developed. However, this method is labor-intensive and requires expensive equipment.
For the diagnosis of many infectious diseases, a serological method is used – the indirect hemagglutination assay (IHA). The essence of the method is that the reaction occurs when immune serum corresponding to the antigen is added to erythrocytes sensitized with antigen, i.e., carrying adsorbed antigen. This reaction often surpasses other serological methods in sensitivity and specificity, and is used for infections caused by bacteria and rickettsiae.
The article presents the results of studies on obtaining specific components for staging the indirect hemagglutination assay in animals with anaerobic enterotoxemia. For the manufacture of erythrocyte diagnosticum, toxoids and soluble antigens intended for sensitization of formalized ram erythrocytes, as well as positive and negative blood serum of rabbits to antigens of bacteria Cl. perfringens serotypes A, B, C, D, E were obtained.
References
Антонов, Б. И. Лабораторные исследования в ветеринарии. Бактериальные инфекции: справочник / Б. И. Антонова. -- М.: Αгропромиздат, 1986. -- 352 с.
Иванова, С. В. Получение эритроцитарного сибиреязвенного антигена / С. В. Иванова, Л. А. Мельникова, Х. Н. Макаев [и др.] // Ветеринарный врач, -- 2019. -- № 2. -- С. 22-25.
Куриленко, А. Н. Бактериальные и вирусные болезни молодняка сельскохозяйственных животных / А. Н. Куриленко, В. Л. Крупальник, Н. В. Пименов. -- М.: КолосС, 2006. -- 296 с.
Ургуев, К. Р. Клостридиозы животных / К. Р. Ургуев. -- М.: Россельхозиздат, 1987. -- 183 с.
Патент № 2625031 Российской Федерации, МПК C12R1/645 (2017.07). Иммуноферментная тест-система для серологической диагностики анаэробной энтеротоксемии животных и контроля напряженности поствакцинального иммунитета: № 2016133320: заявл. 11.08.2016: опубл. 11.07.2017 / Спиридонов А. Г., Никитин А. И., Спиридонов Г. Н., Чернов А. Н., Макаев Х. Н. -- 6 с.
Салимов, В. А. Некоторые особенности патологоанатомической диагностики анаэробной энтеротоксемии телят, вызванной Cl. perfringens типа А / В. А. Салимов, Н. П. Салимова // Актуальные проблемы болезней молодняка в современных условиях: мат. Науч. - практ. конф. -- Воронеж, 2002. -- С. 527-528.
Спиридонов, Г. Н. Инфекционная энтеротоксемия молодняка сельскохозяйственных животных в регионе Среднего Поволжья и Предуралья / Г. Н. Спиридонов, А. Ф. Махмутов, М. Т. Хурамшина, А. Г. Спиридонов // Актуальные вопросы ветеринарной медицины Сибири: мат. науч. - практ. конф. -- Краснообск, 2010. -- С. 134-139.
Хаиров, С. Г. Изыскание способа индикации Clostridium perfringens / С. Г. Хаиров, Д. М. Рамазанова, Ю. С. Салихов [и др.] // Вестник ветеринарии. -- 2009. -- № 48 (1) -- С. 48-50.
Kazuaki, M. Enterotoxigenic Clostridium perfringens: Detection and Identification / M. Kazuaki, L. Jihong, B. A. McClane // Microbes Environ. -- 2012. -- Vol. 27 (4). -- Р. 343-349.
Mauricio A. Navarro. Mechanisms of Action and Cell Death Associated with Clostridium perfringens Toxins / Mauricio A. Navarro, Bruce A. McClane, Francisco A. Uzal // Toxins (Basel). -- 2018. -- Vol. 10(5). -- Р. 212.
Shrestha, A. Enterotoxic Clostridia: Clostridiun perfringens Enteric Diseases / A. Shrestha, F. A. Uzal, B. A. Mc Clane // Microbiology Spectrum. -- 2018. -- Vol. 6(5). -- Р. 1-12.
Spiridonov, G. N. The development of preparations for specific prevention and treatment of anaerobic enterotoxaemia and escherichiosis in calves / G. N. Spiridonov, A. I. Nikitin, H. N. Makaev [et al.] // Bali Medical Journal. -- 2017, Vol. 6 (26). -- P. 124-128.
Antonov, B. I. Laboratory research in veterinary medicine. Bacterial infections: reference book / B. I. Antonova. -- M.: Agropromizdat, 1986. -- 352 p.
Ivanova, S. V. Obtaining erythrocyte anthrax antigen / S. V. Ivanova, L. A. Melnikova, Kh. N. Makaev [et al.] // Veterinary Doctor, -- 2019. -- No. 2. -- P. 22-25.
Kurilenko, A. N. Bacterial and viral diseases of young farm animals / A. N. Kurilenko, V. L. Krupalnik, N. V. Pimenov. -- M.: KolosS, 2006. -- 296 p.
Urguev, K. R. Clostridiosis of animals / K. R. Urguev. -- M.: Rosselkhozizdat, 1987. -- 183 p.
Patent No. 2625031 of the Russian Federation, IPC C12R1/645 (2017.07). Enzyme-linked immunosorbent assay test system for serological diagnosis of anaerobic enterotoxemia in animals and monitoring of post-vaccination immunity intensity: No. 2016133320: appl. 11.08.2016: publ. 11.07.2017 / Spiridonov A. G., Nikitin A. I., Spiridonov G. N., Chernov A. N., Makaev Kh. N. -- 6 p.
Salimov, V. A. Some features of pathoanatomical diagnosis of anaerobic enterotoxemia in calves caused by Cl. perfringens type A / V. A. Salimov, N. P. Salimova // Actual problems of diseases of young animals in modern conditions: proceedings of Scientific and Practical Conf. -- Voronezh, 2002. -- P. 527-528.
Spiridonov, G. N. Infectious enterotoxemia of young farm animals in the Middle Volga and Cis-Urals region / G. N. Spiridonov, A. F. Makhmutov, M. T. Khuramshina, A. G. Spiridonov // Current issues of veterinary medicine in Siberia: proceedings of scientific and practical conf. -- Krasnoobsk, 2010. -- P. 134-139.
Khairov, S. G. Search for a method of indication of Clostridium perfringens / S. G. Khairov, D. M. Ramazanova, Yu. S. Salikhov [et al.] // Bulletin of Veterinary Medicine. -- 2009. -- No. 48 (1) -- P. 48-50.
Kazuaki, M. Enterotoxigenic Clostridium perfringens: Detection and Identification / M. Kazuaki, L. Jihong, B. A. McClane // Microbes Environ. -- 2012. -- Vol. 27 (4). -- P. 343-349.
Mauricio A. Navarro. Mechanisms of Action and Cell Death Associated with Clostridium perfringens Toxins / Mauricio A. Navarro, Bruce A. McClane, Francisco A. Uzal // Toxins (Basel). -- 2018. -- Vol. 10(5). -- P. 212.
Shrestha, A. Enterotoxic Clostridia: Clostridiun perfringens Enteric Diseases / A. Shrestha, F. A. Uzal, B. A. Mc Clane // Microbiology Spectrum. -- 2018. -- Vol. 6(5). -- P. 1-12.
Spiridonov, G. N. The development of preparations for specific prevention and treatment of anaerobic enterotoxaemia and escherichiosis in calves / G. N. Spiridonov, A. I. Nikitin, H. N. Makaev [et al.] // Bali Medical Journal. -- 2017, Vol. 6 (26). -- P. 124-128.
