EPIZOOTOLOGY, GENETICS AND EVOLUTION OF PORCINE CIRCOVIRUS TYPE 2 IN CHINA
УДК: 619:616.98:578.8:636.4(510) DOI: 10.31588/2413_4201_1883_2_258_237
Keywords:
porcine circovirus type 2, genotype, genetic diversity, evolution, Cap proteinAbstract
The genotypic subtype of porcine circovirus type 2 (PCV2) continues to evolve, complicating the epizootic situation. To elucidate the nature of genotype drift in the evolution of PCV2 strains in China, 2529 fully coded ORF2 genes from the NCBI database collected in China from 2004 to 2023 were classified by year of isolation. The results showed that there were two genotype drifts in PCV2 evolution in China over the past 20 years, with genotypes drifting from PCV2a before 2004. A total of 43 strains were further investigated between 2018 and 2023 from three provinces in Northeast China - Liaoning, Heilongjiang and Jilin - which are adjacent to Russia. Among them, PCV2a genotype accounted for 18.6 %, PCV2b genotype accounted for 27.9 %, and PCV2d genotype accounted for 53.4 %. This indicates that PCV2 genetic diversity increased in Northeast China, and PCV2b and PCV2d became the major genotypes prevalent during this period. Comparison of the amino acids of the ORF2 gene encoded by the sequence of virulent strains showed that there were five major regions of variation in the Cap protein among the virulent strains. These residues included amino acid sites 51-91, 121-151, 183-191, 206-217, and 232-234.
References
Обзор по цирковирусной инфекции свиней / В. М. Попова, В. А. Богомолова, Е. В. Маркова, Е. Н. Фелоров, Л. С. Люлькова // Свиноводство. -- 2020. -- № 3. -- С. 8-10.
Орлянкин, Б. Г. Цирковирусные болезни свиней: распространение, диагностика и специфическая профилактика / Б. Г. Орлянкин // Ветеринария. -- 2013. -- № 8. -- С. 3-9.
Стаффорд, В. В. Цирковирусная инфекция свиней. Обзорные данные / В. В. Стаффорд, Я. Б. Стрельцова, М. А. Аноятбеков // Труды ВИЭВ. -- Т. 80. -- № 1. -- С. 324-330. - DOI: 10.30917/ATT-PRINT-2018-1.
Фу Юйцзе, Ф. Ю. Цирковирусная инфекция свиней / Ф. Ю. Фу Юйцзе, С. В. Теребова // Аграрный вестник Приморья. -- 2021. -- № 3(23). -- С. 44-49.
Цирковирусные инфекции возникают во всех свиноводческих хозяйствах: по материалам компании MSD Animal Health. - Текст электронный // Agrovesti.net [сайт]. -- 2016. -- URL: https://agrovesti.net/lib/tech/pig-breeding-tech/tsirkovirusnye-infektsii-voznikayut-vo-vsekh-svinovodcheskikh-khozyajstvakh.html
Differences in virulence among porcine circovirus type 2 isolates are unrelated to cluster type 2a or 2b and prior infection provides heterologous protection / T. Opriessnig, S. Ramamoorthy, D. M. Madson [et al.]. // J/ Gen Viro. -- 2008. -- V. 89. -- P. 2482-2491. -- doi: 10.1099/vir.0.2008/001081-0.
Differential recognition of the ORF2 region in a complete genome sequence of porcine circovirus type 2 (PCV2) isolated from boar bone marrow in Korea / C. H. Kweon, L. T. Nguyen, M. S. Yoo, S. W. Kang // Gene. -- 2015. -- V.569. -- P. 308-312. -- doi: 10.1016/j.gene.2015.04.055.
Genetic analysis of porcine circovirus type 2 in China / C. G. Jiang, G. Wang, Y. B. Tu [et al.]. // Arch Virol. -- 2017. -- V.162. -- P. 2715-2726. -- doi: 10.1007/s00705-017-3414-1.
Genetic characterisation of Porcine circovirus type 2 (PCV2) strains from feral pigs in the Brazilian Pantanal: an opportunity to reconstruct the history of PCV2 evolution / G. Franzo, M. Cortey, A. M. de Castro [et al.] // Vet Microbiol. -- 2015. -- V.178. -- P. 158-162. -- doi: 10.1016/j.vetmic.2015.05.003.
Genetic diversity of porcine circovirus type 2 (PCV2) in Thailand during 2009-2015 / N. Thangthamniyom, P. Sangthong, P. Poolperm [et al.] // Vet Microbiol. -- 2017. -- V. 208. -- P. 239-246. -- doi: 10.1016/j.vetmic.2017.08.006.
Genetic variation analysis of Chinese strains of porcine circovirus type 2 / F. Wang, X. Guo, X. Ge [et al.] // Virus Res. -- 2009. -- V.145. --P. 151-156. -- doi: 10.1016/j.virusres.2009.05.015.
Genotypic shift of porcine circovirus type 2 from PCV-2a to PCV-2b in Spain from 1985 to 2008 / M. Cortey, E. Pileri, M. Sibila [et al.] // Vet J. -- 2011. -- V. 187(3). -- P. 363-368. -- doi: 10.1016/j.tvjl.2009.12.023.
Larochelle, R. Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) strains from cases presenting various clinical conditions / R. Larochelle, R. Magar, S. D'Allaire // Virus Res. -- 2002. -- V. 90. -- P. 101-112. -- doi: 10.1016/s0168-1702(02)00141-7.
Olvera, A. Molecular evolution of porcine circovirus type 2 genomes: phylogeny and clonality / A. Olvera, M. Cortey, J. Segales // Virology. -- 2007. -- V.35. -- P. 175-185. -- doi: 10.1016/j.virol.2006.07.047.
PCV2d-2 is the predominant type of PCV2 DNA in pig samples collected in the U.S. during 2014-2016 / C. T. Xiao, K. M. Harmon, P. G. Halbur, T. Opriessnig. // Vet Microbiol. -- 2016. -- V.197. -- P. 72-77. -- doi: 10.1016/j.vetmic.2016.11.009.
Phylodynamic analysis of porcine circovirus type 2 reveals global waves of emerging genotypes and the circulation of recombinant forms / G. Franzo, M. Cortey, J. Segales [et al.] // Mol Phylogenet Evol. -- 2016. -- V.10. -- P. 269-280. -- doi: 10.1016/j.ympev.2016.04.028.
Pigs naturally exposed to porcine circovirus type 2 (PCV2) generate antibody responses capable to neutralise PCV2 isolates of different genotypes and geographic origins / S. Kurtz, L. Grau-Roma, M. Cortey [et al.] // Vet Res. -- 2014. -- V.45 (1). -- P. 29. -- doi: 10.1186/1297-9716-45-29.
The emergence of a new strain of porcine circovirus-2 in Ontario and Quebec swine and its association with severe porcine circovirus associated disease-2004-2006 / S. Carman, H. Y. Cai, J. DeLay [et al.] // Can J Vet Res. -- 2008. -- V. 72(3). -- P. 259-68.
The emergence of porcine circovirus 2b genotype (PCV-2b) in swine in Canada / C. A. Gagnon, D. Tremblay, P. Tijssen [et al.] // Can Vet J. -- 2007. -- V. 48(8). -- P. 811-819.
Trible, B. R. Genetic variation of porcine circovirus type 2 (PCV2) and its relevance to vaccination, pathogenesis and diagnosis / B. R. Trible, R. R. Rowland. // Virus Res. -- 2012. -- V. 164. -- P. 68-77. -- doi: 10.1016/j.virusres.2011.11.018.
Xiao, C. T. Global molecular genetic analysis of porcine circovirus type 2 (PCV2) sequences confirms the presence of four main PCV2 genotypes and reveals a rapid increase of PCV2d / C. T. Xiao P. G. Halbur, T. Opriessnig // J Gen Virol. -- 2015. -- V.96(Pt7). -- P.1830-41. -- doi: 10.1099/vir.0.000100. Epub 2015 Feb 23.
Review of porcine circovirus infection / V. M. Popova, V. A. Bogomolova, E. V. Markova, E. N. Felorov, L. S. Lyulkova // Pig breeding. -- 2020. -- No. 3. -- P. 8-10.
Orlyankin, B. G. Circovirus diseases of pigs: distribution, diagnosis and specific prevention / B. G. Orlyankin // Veterinary Medicine. -- 2013. -- No. 8. -- P. 3-9.
Stafford, V. V. Circovirus infection of pigs. Review data / V. V. Stafford, Ya. B. Streltsova, M. A. Anoyatbekov // Proceedings of VIEV. -- Vol. 80. -- No. 1. -- P. 324-330. - DOI: 10.30917/ATT-PRINT-2018-1.
Fu Yujie, F. Yu. Circovirus infection of pigs / F. Yu. Fu Yujie, S. V. Terebova // Agrarian Bulletin of Primorye. -- 2021. -- No. 3(23). -- P. 44-49.
Circovirus infections occur in all pig farms: based on materials from MSD Animal Health. - Text electronic // Agrovesti.net [website]. -- 2016. -- URL: https://agrovesti.net/lib/tech/pig-breeding-tech/tsirkovirusnye-infektsii-voznikayut-vo-vsekh-svinovodcheskikh-khozyajstvakh.html
Differences in virulence among porcine circovirus type 2 isolates are unrelated to cluster type 2a or 2b and prior infection provides heterologous protection / T. Opriessnig, S. Ramamoorthy, D. M. Madson [et al.]. // J/ Gen Viro. -- 2008. -- V. 89. -- P. 2482-2491. -- doi: 10.1099/vir.0.2008/001081-0.
Differential recognition of the ORF2 region in a complete genome sequence of porcine circovirus type 2 (PCV2) isolated from boar bone marrow in Korea / C. H. Kweon, L. T. Nguyen, M. S. Yoo, S. W. Kang // Gene. -- 2015. -- V.569. -- P. 308-312. -- doi: 10.1016/j.gene.2015.04.055.
Genetic analysis of porcine circovirus type 2 in China / C. G. Jiang, G. Wang, Y. B. Tu [et al.]. // Arch Virol. -- 2017. -- V.162. -- P. 2715-2726. -- doi: 10.1007/s00705-017-3414-1.
Genetic characterisation of Porcine circovirus type 2 (PCV2) strains from feral pigs in the Brazilian Pantanal: an opportunity to reconstruct the history of PCV2 evolution / G. Franzo, M. Cortey, A. M. de Castro [et al.] // Vet Microbiol. -- 2015. -- V.178. -- P. 158-162. -- doi: 10.1016/j.vetmic.2015.05.003.
Genetic diversity of porcine circovirus type 2 (PCV2) in Thailand during 2009-2015 / N. Thangthamniyom, P. Sangthong, P. Poolperm [et al.] // Vet Microbiol. -- 2017. -- V. 208. -- P. 239-246. -- doi: 10.1016/j.vetmic.2017.08.006.
Genetic variation analysis of Chinese strains of porcine circovirus type 2 / F. Wang, X. Guo, X. Ge [et al.] // Virus Res. -- 2009. -- V.145. --P. 151-156. -- doi: 10.1016/j.virusres.2009.05.015.
Genotypic shift of porcine circovirus type 2 from PCV-2a to PCV-2b in Spain from 1985 to 2008 / M. Cortey, E. Pileri, M. Sibila [et al.] // Vet J. -- 2011. -- V. 187(3). -- P. 363-368. -- doi: 10.1016/j.tvjl.2009.12.023.
Larochelle, R. Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) strains from cases presenting various clinical conditions / R. Larochelle, R. Magar, S. D'Allaire // Virus Res. -- 2002. -- V. 90. -- P. 101-112. -- doi: 10.1016/s0168-1702(02)00141-7.
Olvera, A. Molecular evolution of porcine circovirus type 2 genomes: phylogeny and clonality / A. Olvera, M. Cortey, J. Segales // Virology. -- 2007. -- V.35. -- P. 175-185. -- doi: 10.1016/j.virol.2006.07.047.
PCV2d-2 is the predominant type of PCV2 DNA in pig samples collected in the U.S. during 2014-2016 / C. T. Xiao, K. M. Harmon, P. G. Halbur, T. Opriessnig. // Vet Microbiol. -- 2016. -- V.197. -- P. 72-77. -- doi: 10.1016/j.vetmic.2016.11.009.
Phylodynamic analysis of porcine circovirus type 2 reveals global waves of emerging genotypes and the circulation of recombinant forms / G. Franzo, M. Cortey, J. Segales [et al.] // Mol Phylogenet Evol. -- 2016. -- V.10. -- P. 269-280. -- doi: 10.1016/j.ympev.2016.04.028.
Pigs naturally exposed to porcine circovirus type 2 (PCV2) generate antibody responses capable to neutralise PCV2 isolates of different genotypes and geographic origins / S. Kurtz, L. Grau-Roma, M. Cortey [et al.] // Vet Res. -- 2014. -- V.45 (1). -- P. 29. -- doi: 10.1186/1297-9716-45-29.
The emergence of a new strain of porcine circovirus-2 in Ontario and Quebec swine and its association with severe porcine circovirus associated disease-2004-2006 / S. Carman, H. Y. Cai, J. DeLay [et al.] // Can J Vet Res. -- 2008. -- V. 72(3). -- P. 259-68.
The emergence of porcine circovirus 2b genotype (PCV-2b) in swine in Canada / C. A. Gagnon, D. Tremblay, P. Tijssen [et al.] // Can Vet J. -- 2007. -- V. 48(8). -- P. 811-819.
Trible, B. R. Genetic variation of porcine circovirus type 2 (PCV2) and its relevance to vaccination, pathogenesis and diagnosis / B. R. Trible, R. R. Rowland. // Virus Res. -- 2012. -- V. 164. -- P. 68-77. -- doi: 10.1016/j.virusres.2011.11.018.
Xiao, C. T. Global molecular genetic analysis of porcine circovirus type 2 (PCV2) sequences confirms the presence of four main PCV2 genotypes and reveals a rapid increase of PCV2d / C. T. Xiao P. G. Halbur, T. Opriessnig // J Gen Virol. -- 2015. -- V.96(Pt7). -- P.1830-41. -- doi: 10.1099/vir.0.000100. Epub 2015 Feb 23.
