STR-genotyping of sturgeon fish grown in the RAS: significance and prospects of application
УДК: 639.371/.374; 577.21 DOI: 10.31588/2413_4201_1883_2_255_116
Keywords:
fish, sturgeon, genotyping, STR, RASAbstract
Genetic identification of sturgeon fish in industrial aquaculture is necessitated by the need to form pairs for reproduction, since breeding sturgeon in recirculating aquaculture systems (RAS) carries a risk of inbreeding, leading to a decrease in productivity and fecundity of fish. To study the genetic structure of sturgeon fish, microsatellites are used as genetic markers, due to their wide distribution throughout the genome, and serve as a tool for highly efficient genetic mapping and characterization of taxa, establishing phylogenetic relationships, clarifying conspecific hybridizations, and identifying stocking material.
The article presents the results of studies on the assessment of polymorphism in 7 microsatellite DNA loci of sturgeon fish, in which 69 alleles were identified. All the studied individuals were heterozygous, respectively, the level of observed heterozygosity was 1. Based on the results of the evaluation of STR-loci polymorphism in Siberian sturgeons grown in the RAS, it was found that in the study group, the ratio of expected and observed heterozygosity, as well as the index of fixation (Fis), indicates an excess of heterozygotes in them and a high "reserve" of genetic diversity in STR-loci.
References
Барминцева, А. Е. Использование микросателлитных локусов для установления видовой принадлежности осетровых (Acipenseridae) и выявления особей гибридного происхождения / А. Е. Барминцева, Н. С. Мюге // Генетика. -- 2013. -- Т. 49. -- № 9. -- С. 1093-1105.
Козлова, Н. Применение молекулярно-генетических исследований в аквакультуре осетровых рыб / Н. Козлова, Н. Базелюк, Д. Файзулина, Е. Стоногина // Вестник Астраханского государственного технического университета. Серия: Рыбное хозяйство. -- 2013. -- №. 3. -- С. 113-117.
Мюге, Н.С. Полиморфизм контрольного региона митохондриальной ДНК восьми видов осетровых и разработка системы ДНК-идентификации видов / Н. С. Мюге, А. Е. Барминцева, С. М. Расторгуев [и др.] // Генетика. 2008. -- Т. 44. -- С. 1-7.
Чебанов, М. С. Формирование генетической коллекции осетровых в южном филиале ФГУП ФСГЦР / М. С. Чебанов // Материалы Первой Всероссийской конференции «Генетика, селекция и воспроизводство рыб», СПб, 2002. -- С. 73-80.
Шалгимбаева, Г.М. Генетическое разнообразие севрюги реки Урал / Г. М. Шалгимбаева, А. Е. Барминцева, Л. Н. Мюге, К. Б. Исбеков, Н. С. Мюге // Труды ВНИРО, 2018. -- Т. 171. -- С. 95-105.
Barmintseva, A. E. Natural genetic polymorphism and phylogeography of Siberian sturgeon Acipenser baerii Brandt, 1869 / A. E. Barmintseva, N. S. Myuge, // Russian Journal of Genetics, 2017. -- V. 53(3). -- Р. 358-368. DOI: 10.1134/S1022795417030024
Georgescu, S. E. Analysis of the microsatellite variation in the common hybrid between russian sturgeon (Acipenser Gueldenstaedtii) and siberian sturgeon (Acipenser Baerii) from aquaculture / S. E. Georgescu, O. Canareica, A. Dudu, M. Costache // Transylvanian Review of Systematical and Ecological Research. -- 2013. -- V. 15.2. -- Р. 117-124.
Henderson-Arzapalo, A. Novel microsatellite markers for Atlantic sturgeon (Acipenser oxyrinchus) population delineation and broodstock management / A. Henderson-Arzapalo, T. L. King // Molecular Ecology Notes. -- 2002. -- V. 2. -- P. 437-439.
Nazari, S. Analysis of the genetic structure of the Persian sturgeon (Acipenser persicus) populations: Comparison of control region sequencing and PCR-RFLP analysis of mitochondrial DNA / S. Nazari, M. Pourkazemi, M. R. Khoshkholgh // Iranian Journal of Fisheries Sciences. -- 2020. -- V. 19(6). -- Р. 3201-3220.
Nei, M. Accuracy of estimated phylogenetic trees from molecular data. II. Gene frequency data / M. Nei, F. Tajima, Y. Tateno // Journal of Molecular Evolution. -- 1983. -- Vol. 19. -- P. 153-170.
Welsh, A. B. Identification of microsatellite loci in lake sturgeon. Acipencer fulvescens, and their variability in green sturgeon, A. medirostris / A. B. Welsh, M. Blumberg, B. May // Mol. Ecol. Notes, 2003. -- V. 3. -- P. 47-55.
Barmintseva, A. E. Use of microsatellite loci for species identification of sturgeons (Acipenseridae) and detection of hybrid individuals / A. E. Barmintseva, N. S. Myuge // Genetics. -- 2013. -- Vol. 49. -- No. 9. -- P. 1093-1105.
Kozlova, N. Application of molecular genetic research in sturgeon aquaculture / N. Kozlova, N. Bazelyuk, D. Fayzulina, E. Stonogina // Bulletin of the Astrakhan State Technical University. Series: Fisheries. -- 2013. -- No. 3. -- P. 113-117.
Myuge, N.S. Polymorphism of the mitochondrial DNA control region of eight sturgeon species and development of a DNA identification system / N. S. Myuge, A. E. Barmintseva, S. M. Rastorguev [et al.] // Genetics. 2008. -- Vol. 44. -- P. 1-7.
Chebanov, M. S. Formation of a genetic collection of sturgeons in the southern branch of FGUP FSGTsR / M. S. Chebanov // Proceedings of the First All-Russian Conference "Genetics, breeding and reproduction of fish", St. Petersburg, 2002. -- P. 73-80.
Shalgimbayeva, G.M. Genetic diversity of the Ural River sturgeon / G. M. Shalgimbayeva, A. E. Barmintseva, L. N. Myuge, K. B. Isbekov, N. S. Myuge // Proceedings of VNIRO, 2018. -- Vol. 171. -- P. 95-105.
Barmintseva, A. E. Natural genetic polymorphism and phylogeography of Siberian sturgeon Acipenser baerii Brandt, 1869 / A. E. Barmintseva, N. S. Myuge, // Russian Journal of Genetics, 2017. -- V. 53(3). -- P. 358-368. DOI: 10.1134/S1022795417030024
Georgescu, S. E. Analysis of the microsatellite variation in the common hybrid between russian sturgeon (Acipenser Gueldenstaedtii) and siberian sturgeon (Acipenser Baerii) from aquaculture / S. E. Georgescu, O. Canareica, A. Dudu, M. Costache // Transylvanian Review of Systematical and Ecological Research. -- 2013. -- V. 15.2. -- P. 117-124.
Henderson-Arzapalo, A. Novel microsatellite markers for Atlantic sturgeon (Acipenser oxyrinchus) population delineation and broodstock management / A. Henderson-Arzapalo, T. L. King // Molecular Ecology Notes. -- 2002. -- V. 2. -- P. 437-439.
Nazari, S. Analysis of the genetic structure of the Persian sturgeon (Acipenser persicus) populations: Comparison of control region sequencing and PCR-RFLP analysis of mitochondrial DNA / S. Nazari, M. Pourkazemi, M. R. Khoshkholgh // Iranian Journal of Fisheries Sciences. -- 2020. -- V. 19(6). -- P. 3201-3220.
Nei, M. Accuracy of estimated phylogenetic trees from molecular data. II. Gene frequency data / M. Nei, F. Tajima, Y. Tateno // Journal of Molecular Evolution. -- 1983. -- Vol. 19. -- P. 153-170.
Welsh, A. B. Identification of microsatellite loci in lake sturgeon. Acipencer fulvescens, and their variability in green sturgeon, A. medirostris / A. B. Welsh, M. Blumberg, B. May // Mol. Ecol. Notes, 2003. -- V. 3. -- P. 47-55.
