EVALUATION OF MILK PRODUCTIVITY FOR TATARSTAN-TYPE COWS WITH DIFFERENT COMPLEX GENOTYPES BY LIPID METABOLISM GENES

УДК: 575.174.015.3:636.082:636.2 DOI: 10.31588/2413_4201_1883_4_256_145

Authors

  • Lamara M. FSBEI HE «Kazan State Academy of Veterinary Medicine named after N.E. Bauman»
  • Zagidullin L.R. FSBEI HE «Kazan State Academy of Veterinary Medicine named after N.E. Bauman»
  • Akhmetov T.M. 1) FSBEI HE «Kazan State Academy of Veterinary Medicine named after N.E. Bauman»; 2) Separate Structural Division «Institute of Applied Research of the Academy of Sciences of the Republic of Tatarstan»
  • Shaydullin R.R. FSBEI HE «Kazan State Agrarian University»
  • Tyulkin S.V. 1) FSBEI HE «Kazan State Academy of Veterinary Medicine named after N.E. Bauman»; 2) FSBSI «Federal Research Center for Food Systems named after V.M. Gorbatov» of the Russian Academy of Sciences
  • Ravilov R.Kh. 1) FSBEI HE «Kazan State Academy of Veterinary Medicine named after N.E. Bauman»; 2) Separate Structural Division «Institute of Applied Research of the Academy of Sciences of the Republic of Tatarstan»

Keywords:

cow, complex genotype, gene, OLR1, DGAT1, LEP, milk production

Abstract

The aim of the research was to study the effect on dairy productivity of cows of a complex genotype by the loci of lipid metabolism genes, namely: Low-density lipoprotein receptor (OLR1), Diacylglycerol-O-acyltransferase (DGAT1) and Leptin (LEP). The research was carried out in the conditions of the agricultural company "Agrofirma Rassvet" of the Kukmorsky district of the Republic of Tatarstan on the livestock of 79 cows of the Tatarstan type. As a result of molecular genetic research (PCR-RFLP and AS-PCR), the animal population was sorted into groups, taking into account the complex genotype by the loci of the OLR1, DGAT1 and LEP genes. The highest indicators of milk productivity (milk yield, amount of milk fat and protein) were found in Tatarstan-type cows with complex genotypes of lipid metabolism genes (OLR1, DGAT1, LEP), three complex genotypes AC/AA/CC, AC/AA/CT and AC/AK/CC differed. An assessment of the influence of the complex genotype by the loci of the OLR1, DGAT1 and LEP genes of the first heifers on milk productivity indicators is proposed, thanks to which it is possible to increase the volume of high-quality dairy raw materials necessary to increase the efficiency of cattle breeding.

Author Biographies

Lamara M., FSBEI HE «Kazan State Academy of Veterinary Medicine named after N.E. Bauman»

Postgraduate Student

Zagidullin L.R., FSBEI HE «Kazan State Academy of Veterinary Medicine named after N.E. Bauman»

Candidate of Biological Sciences, Associate Professor, Head of Department

Akhmetov T.M., 1) FSBEI HE «Kazan State Academy of Veterinary Medicine named after N.E. Bauman»; 2) Separate Structural Division «Institute of Applied Research of the Academy of Sciences of the Republic of Tatarstan»

Doctor of Biological Sciences, Professor, Head of Department, Senior Researcher

Shaydullin R.R., FSBEI HE «Kazan State Agrarian University»

Doctor of Agricultural Sciences, Associate Professor, Head of Department

Tyulkin S.V., 1) FSBEI HE «Kazan State Academy of Veterinary Medicine named after N.E. Bauman»; 2) FSBSI «Federal Research Center for Food Systems named after V.M. Gorbatov» of the Russian Academy of Sciences

Doctor of Biological Sciences, Senior Researcher

Ravilov R.Kh., 1) FSBEI HE «Kazan State Academy of Veterinary Medicine named after N.E. Bauman»; 2) Separate Structural Division «Institute of Applied Research of the Academy of Sciences of the Republic of Tatarstan»

Doctor of Veterinary Sciences, Professor

References

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Komisarek, J. Effect of ABCG2, PPARGC1A, OLR1 and SCD1 gene polymorphism on estimated breeding values for functional and production traits in Polish Holstein-Friesian bulls / J. Komisarek, Z. Dorynek // J. Appl. Genet. -- 2009. -- V. 50 2. -- P. 125-132.

Liefers, S. C. Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in Holstein heifers / S. C. Liefers, R.F. Veerkamp, T. Vanderlede // J. Dairy Sci. -- 2002. -- V. 85. -- P. 1627-1638.

Mohammed, S. A. DGAT1 gene in dairy cattle Glob / S. A. Mohammed, S. A. Rahamtalla, S. S. Ahmed, [et al.] // J. Anim. Sci. -- 2015. -- V. 3. -- P. 191-198.

Pandey, V. Effect of leptin gene polymorphism on reproduction and production traits in Sahiwal cattle // V. Pandey, R. Nigam, S.P. Singh, [et al.] // Ruminant Science. -- 2017. -- V. 6 (2). -- P. 237-242.

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Tyul'kin, S.V. Polymorphism of Somatotropin, Prolactin, Leptin, and Thyreoglobulin Genes in Bulls / S. V. Tyul'kin, T. M. Akhmetov, E. F. Valiullina, R. R. Vafin // Russian Journal of Genetics: Applied Research. -- 2013. -- V. 3 3. -- P. 222-224.

Tyulkin, S.V. Development of a method for PCR-RFLP on the example of DGAT1 gene in cattle / S. V. Tyulkin, R. R. Vafin, A.V. Muratova, [et al.] // Fundamental research. -- 2015. -- № 2-17. -- P. 3773-3775.

Wayne, J. Neuropeptide: It´s in the neural regulation of reproductive function and feed intake in mammalian species / J. Wayne, W. J. Kuenzel, G. S. Fraley // Poultry and Avian Biology Reviews. -- 1995. -- V. 6. -- P. 185-209.

Anggraeni, A. Genetic polymorphisms of the OLR1 and DGAT1 genes associated with milk components in Holstein Friesian dairy cattle under an intensive management in Central Java / A. Anggraeni // IOP Conference Series: Earth and Environmental Science. -- 2019. -- V. 287. 012001.

Bouwman, A. C. Genome-wide association of milk fatty acids in Dutch dairy cattle / A. C. Bouwman, H. Bovenhuis, M. H. P. W. Visker, J. A. van Arendonk / BMC Genet. -- 2011. -- V. 12. -- P. 1-12.

Cases, S. Identification of a gene encoding an acyl CoA: diacylglycerol acyltransferase, a key enzyme in triglycerol synthesis / S. Cases, S.J. Smith, Y. Zheng, [et al.] // E Proc. Natl. Acad. Sci. USA. -- 1998. -- V. 95. -- P. 13018-13023.

Glantz, M. Effect of polymorphism in the leptin, leptin receptor and acyl-CoA: Diacylglycerol acyltransferase 1 (DGAT1) genes and genetic polymorphism of milk proteins on bovine milk composition / M. Glantz, H.L. Mansson, H. Stalhammar, M. Paulsson // Journal of Dairy Research. -- 2012. -- V. 79. -- P. 110-118.

Grisart, B. Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition / B. Grisart, W. Coppieters, F. Farnir, [et al.] // Genome Res. -- 2020. -- V. 12. -- Р. 222-231.

Khatib, H. Association of the OLR1 gene with milk composition in Holstein dairy cattle / H. Khatib, S. D. Leonard, V. Schutzkus, [et al.] // J. Dairy Sci. -- 2006. -- V. 89. -- P. 1753-1760.

Komisarek, J. Effect of ABCG2, PPARGC1A, OLR1 and SCD1 gene polymorphism on estimated breeding values for functional and production traits in Polish Holstein-Friesian bulls / J. Komisarek, Z. Dorynek // J. Appl. Genet. -- 2009. -- V. 50 2. -- P. 125-132.

Liefers, S. C. Associations between leptin gene polymorphisms and production, live weight, energy balance, feed intake, and fertility in Holstein heifers / S. C. Liefers, R.F. Veerkamp, T. Vanderlede // J. Dairy Sci. -- 2002. -- V. 85. -- P. 1627-1638.

Mohammed, S. A. DGAT1 gene in dairy cattle Glob / S. A. Mohammed, S. A. Rahamtalla, S. S. Ahmed, [et al.] // J. Anim. Sci. -- 2015. -- V. 3. -- P. 191-198.

Pandey, V. Effect of leptin gene polymorphism on reproduction and production traits in Sahiwal cattle // V. Pandey, R. Nigam, S.P. Singh, [et al.] // Ruminant Science. -- 2017. -- V. 6 (2). -- P. 237-242.

Sawamura, T. An endothelial receptor for oxidized low-density lipoprotein / T. Sawamura, N. Kume, T. Aoyama, [et al.] // Nature. -- 1997. -- V. 386. -- P. 73-77.

Tyul'kin, S.V. Polymorphism of Somatotropin, Prolactin, Leptin, and Thyreoglobulin Genes in Bulls / S. V. Tyul'kin, T. M. Akhmetov, E. F. Valiullina, R. R. Vafin // Russian Journal of Genetics: Applied Research. -- 2013. -- V. 3 3. -- P. 222-224.

Tyulkin, S.V. Development of a method for PCR-RFLP on the example of DGAT1 gene in cattle / S. V. Tyulkin, R. R. Vafin, A.V. Muratova, [et al.] // Fundamental research. -- 2015. -- № 2-17. -- P. 3773-3775.

Wayne, J. Neuropeptide: It´s in the neural regulation of reproductive function and feed intake in mammalian species / J. Wayne, W. J. Kuenzel, G. S. Fraley // Poultry and Avian Biology Reviews. -- 1995. -- V. 6. -- P. 185-209.

Published

2026-08-21

Issue

Vol. 256 No. 4 (2023): SCIENTIFIC NOTES OF THE KAZAN STATE ACADEMY OF VETERINARY MEDICINE NAMED AFTER N.E. BAUMAN NO. 4 (256) 2023

Section

VETERINARY MEDICINE

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