PROTECTIVE EFFECT OF A PROBIOTIC ON MORPHOFUNCTIONAL INDICATORS OF THE LIVER OF BROILER CHICKENS WITH ENROFLOXACIN-INDUCED HEPATOPATHY
УДК: 633.313; 631.461.52 DOI: 10.31588/2413_4201_1883_1_265_32
Keywords:
probiotic, enrofloxacin, hepatoprotective effect, broiler chickens, morphofunctional indicators of liver, histology, antibiotic alternatives, poultry farming.Abstract
The aim of the study was to evaluate the protective effect of a probiotic based on Bacillus subtilis BKM B3701D on morphofunctional indicators of the liver of broiler chickens with enrofloxacin-induced hepatopathy. The experiment was carried out on 100 broiler chickens of Ross-308 line from day 1 to day 21 of life. Birds were divided into 4 groups: control (standard diet), probiotic (probiotic administered daily from day 1 to day 20 at a dose of 1 g/kg of body weight), enrofloxacin (200 mg/l of water for the first 5 days), enrofloxacin + probiotic (enrofloxacin for the first 5 days, followed by probiotic from day 6 to day 20). On 1, 7, 14 and 21 days of life birds were slaughtered, body and liver weights were determined and liver histology was performed using hematoxylin and eosin staining. It was found that enrofloxacin causes centrilobular hepatic hyperemia of liver of 14-day-old chickens (detected in 50% of histological preparations). The frequency of hyperemia decreased to 28.6% and its severity was moderate in the group with the combined use of an antibiotic and subsequent probiotic. Morphological changes in all groups leveled out by the 21st day of life. Live weight in the enrofloxacin group on the 14th day was lower than the control (479.02 ± 18.10 g versus 507.72 ± 7.71 g, p < 0.05), but differences disappeared by the 21st day. Live weight was reduced on the 21st day in the enrofloxacin + probiotic group (882.21 ± 24.28 g versus 968.74 ± 21.49 g in the control, p < 0.001), which may be associated with the redistribution of resources to reparative processes in the liver. The obtained results indicate the hepatoprotective potential of Bacillus subtilis BKM B3701D probiotic, which is capable of reducing microcirculatory disorders caused by enrofloxacin and justify its inclusion in broiler growing regimens to reduce the drug load on the liver.
