MODIFYING EFFECT OF WEAK BROADBAND ELECTROMAGNETIC FIELDS (EMF) ON THE PROCESS OF B16 MELANOMA METASTASIS IN C57BL/6 MICE

УДК: 591.29:615.8:616.006 DOI: 10.31588/2413_4201_1883_1_265_59

Abstract

The resistance of skin melanoma to standard drug therapy regimens necessitates the search for new treatment approaches. Therefore, studying the influence of physical factors, particularly weak non-ionizing radiation, on tumor growth processes is a relevant direction. The aim of this study was to investigate the metastasis of experimental B16 melanoma in C57BL/6 mice exposed to weak non-ionizing non-thermal pulsed electromagnetic radiation. The experiment was conducted at Far Eastern State Agrarian University. The study included 120 8-week-old male mice, divided into two groups (n=60). The animals were inoculated intradermally in the right lateral region with 1 million B16 murine melanoma cells. No specific antitumor therapy was administered to either group. Mice in the experimental group were exposed to TOR apparatus daily for 60 minutes starting on the 9th day after tumor transplantation. The control group did not receive similar procedures. The size and location of metastases in regional lymph nodes and distant organs were assessed using X-ray and ultrasound. Statistically significant differences in the maximum linear dimensions of metastases between the groups were recorded on days 10, 21 and 29. In the control group, metastasis to lung tissue was recorded as early as day 7. In the experimental group, the first signs of lung damage appeared only on the 22nd day and by the 52nd day, lung metastases were not detectable radiographically. Ultrasound examination on the 32nd day also revealed no metastases in the peripheral lymph nodes or internal organs in the animals receiving the therapy. Thus, the use of noninvasive broadband electromagnetic therapy significantly slows the rate of metastasis of B16 melanoma to the chest organs and induces regression of metastatic lesions in internal organs.